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学生对 University of California, Irvine 提供的 Basics of Extracellular Vesicles 的评价和反馈

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353 个评分

课程概述

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

热门审阅

MR

Jan 10, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

May 16, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

筛选依据：

51 - Basics of Extracellular Vesicles 的 75 个评论（共 97 个）

创建者 Jess M

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Sep 14, 2016

Aprendí mucho. Muy completo, aunque pudieron haber profundizado un poco más.

创建者 Mario N

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Jan 1, 2022

En efecto, bastante básico e introductorio al campo. Muy útil para iniciar.

创建者 Alaika D

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Jan 10, 2024

A very good explanation of the topic, describing all the necessary points.

创建者 Rafaela S

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Apr 2, 2024

Excellent course. Short classes, with questions to test your knowledge.

创建者 LIN, T

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Mar 1, 2020

Great course to learn the basic knowledges of extracellular vesicles!

创建者 Petr N

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Oct 10, 2020

Very useful course not only for newbies in the EV field. Thank you

创建者 Daniel A S B

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Apr 3, 2022

It´s a great course to people that starting to study of vesicles.

创建者 Asmaa A M A

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May 14, 2020

Excellent course, totally enjoyed it and benefited alot from it

创建者 Laura M M E

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May 4, 2020

Excelente curso, expositores con amplio conocimiento del tema

创建者 Stephanie B

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Oct 5, 2019

Good basic overview for someone starting in their studies

创建者 Wenhao L

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Apr 26, 2017

Very wonderful courses! Better than reading 100 papers!

创建者 Mohammad R

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Feb 5, 2017

This course gives an excellent overview of the field!

创建者 Nahla I S H

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Apr 22, 2020

A very good course, really it was very informative.

创建者 pbmansfield

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Mar 6, 2017

Well put together and very informative. Great job!

创建者 Dr.� S R S A P -

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Jun 25, 2020

very helpful and nice videos and the contents too

创建者 Julia M

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Jan 13, 2023

Great introduction to EV research and methods.

创建者 Ramiro R d l C

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Nov 4, 2020

Great! Very informative and I learned a lot.

创建者 Emilio E S

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Jul 26, 2021

really good course. I learn a lot about EVs

创建者 Regina C

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Dec 29, 2020

Very interesting and simple to understand

创建者 Olivia C

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Aug 6, 2020

very useful course, I really enjoy it!

创建者 Y. M

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Apr 9, 2020

Very good for beginners.

创建者 PASUT L

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Feb 4, 2023

morally excellent

创建者 David H

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Aug 31, 2020

excellent course

创建者 Ábner M N

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Nov 21, 2019

Amazing course!

创建者 Julio C T V

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Aug 15, 2022

Excellent!!!!