Name: Basics of Extracellular Vesicles
Brand: University of California, Irvine
Availability: OnlineOnly
Rating: 4.7 (97 reviews)

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学生对 University of California, Irvine 提供的 Basics of Extracellular Vesicles 的评价和反馈

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353 个评分

课程概述

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

76 - Basics of Extracellular Vesicles 的 97 个评论（共 97 个）

创建者 Dr T S R

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Sep 17, 2020

Super Course

创建者 Muhammad H

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May 14, 2023

Outstanding

创建者 Adel D

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Nov 3, 2016

very good!

创建者 CAROLINA T P

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Jul 30, 2021

Excellent

创建者 ganesha p

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Sep 16, 2020

Excellent

创建者 Lee-Ann M C

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Jul 9, 2021

Usefull

创建者 Kaio M R

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Oct 9, 2017

ÓTIMO

创建者 Emayamsavitha

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Apr 27, 2020

Gud

创建者 PRIYASHREE N

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Jul 3, 2020

The way the course was introduced and was taught is really amazing. There were few issues in understanding the audio and there was problem with subtitles as well but still it was a good and amazing experience to meet and listen to researchers from various parts of world. Though it is a blooming field, there was a lot of information in this course. And I gained lot of information from this course. Thanks for every single researcher and professor who are involved in this course. It was a great pleasure to meet and hear your lectures.

创建者 Carmen M P C

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Sep 14, 2022

La última clase "Nanoparticle tracking analysis" es muy aburrida, debería ser algo más dinámica. Por ejemplo, podrían poner preguntas tipo test entre medias como hicieron con las otras clases. Así te obligas a escuchar.

创建者 Mainak B

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Nov 2, 2019

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.

创建者 Julia B M C

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Sep 27, 2024

Algumas aulas são muito superficiais ou focam em temas que não terão muito uso. Mas no geral é um curso excelente, para ter uma noção básica sobre EVs.

创建者 Tim D R

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May 25, 2022

A lot of information broken down in logical and short segments (30 minutes) with great overviews of what each module will cover.

创建者 Enrique V O

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May 11, 2017

It is good much info about vesicles that you can't get from the books and techniques to study them.

创建者 Shalmali P

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Mar 3, 2018

Very nice nd inormative presentations. But few words in many presentations are not audible.

创建者 Marine H

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Aug 11, 2020

The last two modules were extremely helpful and a very good opener to this topic.

创建者 David d V L

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Mar 29, 2018

Excellents classes I have learned a lot of procotols about EVs

创建者 paula s m c

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Apr 4, 2022

Es un curso muy completo y bien orientado.

创建者 Xiaokun L

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Jul 20, 2017

nice

创建者 Alexander Z

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Oct 7, 2020

Overall, pretty good background information. But some of these lectures need to be a bit more audible. I only had one issue with a quiz in which it felt like the question asked either wasn't covered or emphasized as important to the overall subject.